Cloning of Blue Ternate (Clitoria ternatea) through Tissue Micropropagation
P. Geronimo, K. Graspela, G. Lim, S. Maggay, Z. Pilar, F. Sibug
Quantitative Research, 2023
Abstract
This research paper provides a methodological development and comprehensive understanding of cloning a medicinal plant known as blue ternate (Clitoria ternatea) through the process of tissue micropropagation. This medicinal plant is known to have many health benefits such as boosting brain health, fighting against cancer and lowers blood pressure through utilizing it as a herbal infusion tea by brewing its dried petals. In this study, the process of tissue micropropagation serves as a baseline to execute the cloning of plants and lessen the time needed to grow plants through the traditional process wherein soil, sunlight, and water are the main growth factors that affect the plants. As it served its purpose, the descriptive question revolved around the change of color between the traditionally planted blue ternate and the cloned plant while the inferential questions leaned on the mass, height, pH level, and average growth per day. Previous studies were conducted and one of which made use of Indian snakeroot (Rauvolfia Serpentina), an endangered medicinal plant that was put into a medium called Murashige and Skoog that enabled the increase of the multiplication rate of the shoots of the aforementioned plant. In this paper, the medium utilized was an agar medium wherein the materials were distilled water, agar, and sugar that served as the source of nutrients of the explant that came from the traditionally planted blue ternate. After its solidification, the explant was put in and observed for three weeks. Through the statistical method known as Independent t-test, the researchers were able to get the p-value of each characteristic being looked for and as a result, there were significant differences between the color and mass of the traditionally planted blue ternate and the cloned plant while there are changes on the height, pH level and average growth per day but are not significant. This resulted in the conclusion that the researchers were partially successful in cloning.
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